In 1976, psychiatrist and researcher Michael Russell indicated the following:
"Smokers cannot easily stop smoking because they are addicted to nicotine, People smoke for nicotine but they die from tar."
In 1976, psychiatrist and researcher Michael Russell indicated the following:
"Smokers cannot easily stop smoking because they are addicted to nicotine, People smoke for nicotine but they die from tar."
The extracts from the 3 macera_x0002_tions were pooled, filtered and subjected to a rotary evaporation to obtain crude extracts.
Acid-base extraction is based on the alkaloid property of nicotine, which involves dif_x0002_ferent solubility levels in water and an organic solvent. The 50 g of tobacco leaves was boiled with 750 mL of water at 80 ± 5 ◦C for 20 min, then 10 g of sodium carbonate was added and the mixture was continuously heated for 10 min. After filtration, the obtained filtrate was adjusted to pH 12 using sodium hydroxide and extracted with chloroform (100 mL × 2 times) using the liquid-liquid extraction technique. The chloroform of the filtrate was removed using a rotary evaporator at 50 ◦C under vacuum to obtain crude extracts .
2. Picric Acid Method
1 kg of tobacco leaves are chopped, dried, crushed and sifted to obtain 100 g of dry powder. Add 1 mol/L NaOH solution 0.5 L, stir for 30 min, filtration, before carrying out chloroform extraction and water bath heating recovery of chloroform. The concentrate is dried in a water bath, the residue (oil) is dissolved with a small amount of methanol, and the fluffy yellowish picric acid nicotine precipitates immediately after adding the saturated methanol solution of the picric acid. After precipitation and recrystallization with an aqueous solution of ethanol, about 4.6 g picric acid nicotine crystals can be obtained, with an extraction efficiency of 60%.